Evaluation of phagocytic function of bovine peripheral polymorphonuclear leukocytes by whole blood chemiluminescence
1991
Makimura, S. (Miyazaki Univ. (Japan). Faculty of Agriculture) | Sawaki, M. | Usui, M.
Zymosan-induced and luminol-dependent chemiluminescence of whole blood (whole blood CL) from Holstein heifers was carried out to estimate the phagocytic function of polymorphonuclear leukocytes (PMNs). Whole blood (0.1ml) was examined directly and results were obtained within 20 min. Phagocytic function of PMNs can be estimated from the peak CL of whole blood CL and the number of PMNs in a specimen, and the opsonic activity from the time the peak CL is shown after the addition of non-opsonized zymosan. Most suitable temperatures to keep diluted whole blood for the CL measurement are 10-15degree Centigrade. Then, influences of various scavengers of reactive oxygen species on whole blood CL were examined. Thus, this method offers information concerning the functions of the phagocytic system in whole blood, which is useful for bovine clinical application
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