Expression of recombinant Candida rugosa lipase
1994
Lotti, M. | Brocca, S. | Fusetti, F. | Alberghina, L. (Milano Univ. (Italy). Dipt. di Fisiologia e Biochimica Generali. Sez. Biochimica Comparata)
In the asporogenic yeast Candida rugosa lipase is encoded by a family of genes. As a consequence, multiple lipase isozymes with similar but not identical molecular and catalytic properties are secreted in the culture medium. The gene LIP1, coding for the major lipase protein, was engineered by replacement of its leader sequence and expressed in Saccharomyces cerevisiae under the control of an inducible promoter. Levels of expression as high as 20 mg per litre of glycosylated intracellular protein were obtained.
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