Production of cross-reacting and heterospecific monoclonal antibodies for plant virus detection
1993
Frison, E.A.
In order to study the potential usefulness of cross-reacting and heterospecific monoclonal antibodies (MAbs) for plant virus detection, investigations were carried out with the nepovirus, the carlavirus and the tombusvirus groups. A hybridoma screening technique was applied which involved testing of the culture supernatants, before the hybridomas were cloned to single cell lines for their reaction with a range of heterologous antigens in addition to the homologous antigen. This approach has allowed the selection of MAbs cross-reacting with viruses for which no or little serological relationships had been reported. With nepoviruses, one MAb reacting with arabis mosaic virus (ArMV) and grapevine fanleaf virus (GFLV) and one reacting with ArMV and raspberry ringspot virus (RRSV) were produced. This is the first report of MAbs cross-reacting with these nepoviruses. With the carlavirus group a MAb cross-reacting with six out of nine carlaviruses tested could be screened. With the tombusvirus group, when a fusion peptide corresponding to a conserved sequence of the shell domain of the coat protein of cucumber necrosis virus (CuNV) was used as immunogen, MAbs cross-reacting with the native coat protein of CuNV were produced. This technique also allowed the selection of useful heterospecific MAbs. In a fusion with ArMV as immunogen, five heterospecific MAbs were selected, two reacting specifically with cherry leafroll virus (CLRV), two with RRSV and one with tomato black ring virus (TBRV).
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