Factors improving L-threonine production by a three L-threonine biosynthetic genes-amplified recombinant strain of Brevibacterium lactofermentum
1994
Ishida, M. (Ajinomoto Co. Inc., Kawasaki, Kanagawa (Japan). Central Research Labs.) | Kawashima, H. | Sato, K. | Hashiguchi, K. | Ito, H. | Enei, H. | Nakamori, S.
When a Brevibacterium lactofermentum L-threonine producer that accumulated L-lysine as well, was transformed with a recombinant plasmid carrying the indigenous hom, thrB, and thrC genes, delayed growth and plasmid instability were observed. Addition of organic nutrients, vitamins (thiamine.HCl and d-biotin), and NaCl under the optimal culture conditions solved these problems and further increased threonine production in a small jar fermentor
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