Effect of culture temperature shift on the cellular sugar accumulation of Chlorella vulgaris SO-26
1994
Hosono, H. (Tokyo Gas Co. Ltd., Yokohama (Japan)) | Uemura, I. | Takumi, T. | Nagamune, T. | Yasuda, T. | Kishimoto, M. | Nagashima, H. | Simomura, N. | Natori, M. | Endo, I.
We investigated the effects of culture temperature on the maximum specific growth rate and cellular sugar accumulation, and the effect of a temperature shift on the sugar accumulation of cells of an Antarctic green alga, Chlorella vulgaris S0-26, in a batch culture system. Decreases in temperature appeared to correlate with decreases in the maximum specific growth rate; on the other hand, the cellular sugar content showed a reverse tendency against temperature. We attempted to use this tendency to improve sugar productivity in Chlorella. First, we cultured Chlorella at 20 degrees C during the logarithmic growth phase to obtain a high specific growth rate. The culture temperature was then shifted from 20 degrees C to 14 degrees C during the stationary growth phase to increase the cellular sugar content. As a result, we obtained a 25% increase in sugar production over that obtained by culture at 20 degrees C throughout the culture
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