Molecular genetic studies of mitochondrial genome in rapeseed (Brassica napus L.) in relation to cytoplasmic male-sterility
1993
Handa, H. (National Inst. of Agrobiological Resources, Tsukuba, Ibaraki (Japan))
In order to characterize cytoplasmic male-sterility (cms) in rapeseed plant, molecular genetic studies of mitochondrial genome were carried out. Restriction endonuclease analysis of mitochondrial (mt) DNA revealed the intraspecific variation of the mitochondrial genome. Field experiments showed the classification of cytoplasm based on restriction endonuclease analysis of mitochondrial genome was comparable with that based on the ability to induce male sterility. The results obtained from the data of restriction endonuclease and RELP analyses of mtDNAs suggested the origin and evolution of rapeseed cytoplasm. Southern blot analysis using mitochondrial gene proves revealed a different gene organization around the atp6 locus in cms and normal mtDNAs. Two atp6 locus were examined extensively, and a novel reading frame (pol-urf) was found in cms mitochondria only, which might be co-transcribed with atp6. Pol-urf may have been originated by recombinations among three different regions of mitochondrial genome. There was no variation between RNA editing status of cms and normal atp6 transcripts
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