Fixed-cell immunoperoxidase technique with monoclonal antibodies for the detection of hog cholera virus gpe-strain related antigen
1993
Jinyama, M. (Japan. Ministry of Agriculture, Forestry and Fisheries, Kokubunji, Tokyo. National Veterinary Assay Lab.) | Sugiyama, M. | Inoue, Y. | Taguchi, K. | Ishikawa, K. | Saitoh, A. | Itoh, O. | Muramatsu, M.
Fixed-cell immunoperoxidase technique for the quantitation of hog cholera virus (HCV) GPE-strain was evaluated and compared to the western equine-encephalomyelitis virus (WEEV) interference method commonly used. Primary swine testis cells fixed in periodate-lysine-paraform-aldehyde which were inoculated with the GPE-strain simultaneously on 96-well microplates were used as the antigen. The first sera consisted of diluted 2 monoclonal antibodies against HCV-related cell antigens. There was no significant difference in the virus content of the HCV line vaccine between the fixed-cell immunoperoxidase technique and WEEV interference method (P0.05). These results obtained show that this technique can replace the WEEV interference method as a test of the content of hog cholera live vaccine virus
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