Embryogenic and organogenic callus induction, maintenance, and plantlet regeneration in maize (Zea mays L.) cv IPB Var 4 and inbred line Pi23
1993
Perez, E.A. | Patena, L.F. | Logrono, M.L. (Philippines Univ., Los Banos, College, Laguna (Philippines). Inst. of Plant Breeding)
Media additives: asparagine, L-proline and casein hydrolysate (CH) were tested for embryogenic (E) and organogenic callus induction and maintenance in maize (Zea mays L.) using immature embryos of cultivar IPB var 4 and inbred line Pi23. Best results on callus induction were observed on cultures grown on MN6 medium which consisted of N6 salts and supplemented with 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D), 2.25 mg/L glycine, 1.5 mg/L thiamine HCl, 9.2 mg/L nicotinic acid, 1.5 mg/L pyridoxine HCl, 2 percent sucrose and the media additives, L-proline (2.3 g/L) and casein hydrolysate (200 mg/L). The primary callus obtained was friable and compact. Maintenance of E. calli was achieved by consistent selection of such calli and transfer every 14 days onto fresh medium with increased concentration of 2,4-D (1.0 mg/L). Brief exposure (3-6 days) of E. calli to differentiation medium supplemented with 3-4 mg/L 6-benzylaminopurine (BAP) increased the recovery of germinating embryos. Transfer of plantlets to hormone-free medium allowed their continued development. A progeny (G1) of IPB Var 4 was recovered by pollination of the ear with pollen of cv Yellow Sweet Glutinous from field-grown plants
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