Effects of Gamma irradiation on aseptic culture of Chrysanthemum
1988
Chutinthon Buranakanit
Effect of gamma irradiation on 9 chrysanthemum cultivars were studied. In Vitro culture chrysanthemums were acute irradiated with 0.5 and 1 krad of gamma ray for 1 or 2 times. Then they were subcultured on Murashige and Skoog media supplemented with 20 percent coconut water and 40 percent sugar. With the increasing dose, and exposure time, the survival rate as well as growth rate were decreased. Abnormal characters were found such as plants with many nodes, some with yellow leaves, the others with brown leaves. When plantlets were transplanted into soil, shoot from axillary bud of VM 1 plant were cut to produce VM 2 plants. The diameter of receptacles, flower stem, disc florets, number of ray florets, numbers of disc florets, Shelf life and water consumed were considerably increased. Colors were also variable, some flowers of irradiated plant of Yellow Cappa at 0.5 krad for 1 or 2 times were changed from Yellow to bronze. At the same dose in Ping Pong were changed from white to pale pink. Ta-Shin-Hon at 0.5 krad 1 times were changed from purple to purple-brown and Murera (white) at 1 krad and 0.5 krad 1 or 2 times changed colors from white to yellow, pink, light brown and strip of light brown on pink ray florets.
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