Formation and regeneration of Agaricus bitorquis protoplasts
1988
Upatham Pongpara | Preecha Klingesorn (Mahidol Univ., Bangkok (Thailand). Faculty of Science. Dept. of Biology) | Sanchai Tantrayaporn
Interest in cultivation of Agaricus bitorquis arose due to its many valuable characteristics like resistance to virus and production of white fruiting bodies of better shelf life, which make its cultivation interesting as well as due to its optimal cropping temperature that coincides with hot temperature of Thailand. In order to produce the suitable strain for Thai climate, attempt to interbreed between A. bitorquis and A. bisporus has been made by protoplast fusion technique. Several factors to maximize the yield of protoplasts have been examined. Four osmotic stabilizer had been tested, the best one was 0.6 M mannitol at pH 6.8, Four enzymes; cellulase, hemicellulase, chitinase and Novozyme 234 were tested for maximal cell wall digestion. 1 % Novozyme 234 at incubation time 44 hrs gave the maximum yield of protoplast. The malt extract medium with 0.6 M. mannitol was used to enhance the mycelium regeneration. Intensive research work on suitable condition for formation and regeneration of this mushroom are in progress.
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