Image data analysis of single barley [Hordeum vulgare] coleoptile cell, 1: Evaluation of cytoplasmic streaming
1994
Kameoka, T. (Mie Univ., Tsu (Japan). Faculty of Bioresources) | Horibe, K.
A cytoplasmic activity was estimated by the silicon droplet movement, which had been injected into an inoculated host cell, induced by the cytoplasmic streaming. In this paper, a digital image processing system was constructed for tracing the oil droplet movement within a target cell. Image data of a single barley coleoptile cells, recorded with a time-lapse video tape recorder, were used. A finite difference filter was effective in finding the blurred edge of the droplet. Also blue image data was used for the treatment because a less density variance of the background image was confirmed. It was found that the velocity of a cyto-plasmic streaming was about 0 to 50 micro-meter/min before injection, while it was about 0 to 100 micro-meter/min after the injection. These results agreed well with those measured by eyes
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