Regulation of molecular structure of acid-dispersed collagen with alkaline pretreatment
1994
Nakazaki, S. (Hokuyo Co. Ltd., Yamagata (Japan)) | Takaku, K. | Yuguchi, M. | Edamatsu, H. | Takahashi, K.
Corium pieces obtained from steer hide were pretreated with sodium hydroxide at 25 degrees C for 0-10 days and dispersed mechanically in lactic acid solution, resulting in the preparation of soluble collagen. The acid-dispersed collagen (ADC) obtained was a substantially pure type I collagen which had a large degree of intermolecular cross-linking compared with pepsin-solubilized collagen. The alkaline pretreatment lowered pI (5.4-5.0) and the denaturation temperature (34-30 degrees C) of ADC, while the intermolecular cohesion increased in homogeneity. There fore, these molecular structures can be regulated by controlling alkaline pretreatment
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