Manipulating cassava cyanogenesis in tissue culture by using extrinsic factors
1995
Panutat, P. | Sanghirun, S. | Limpaseni, T. | Chulavatnatol, M.
Together with genetics, environmental factors exert strong influence on the cyanide content of cassava roots. To create acyanogenic varieties of cassava, genetic engineering still requires much more investment. But better understanding of the extrinsic factors that control cyanogenesis may result in alternatives that are practical to apply for managing the cyanide content of cassava roots. To this end, cassava plantlets in tissue culture were first shown to accumulate linamarin during the aseptic growth. This system was then employed to search for and evaluate different extrinsic agents that could interfere with linamarin synthesis. Based on the molecular knowledge of linamarin biosynthesis, several inhibitors of glucosyltransferase and cytochrome P450 were identified and tested in tissue culture for specific suppressive effects on the linamarin level of cassava plantlets. Field trials of these compounds could lead to a practical agro-management of the cassava cyanide problem.
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