A recent method of phytopathogenic fungi taxonomy research
1995
Novosadova, D. (Slovenska Akademia Vied, Ivanka pri Dunaji (Slovakia). Ustav Experimentalnej Fytopatologie a Entomologie)
Recent methods of phytopathogenic fungi taxonomy are discussed. Working on a molecular level means working with nucleic acid, mainly DNA. The isolation of that acid was made by pheno-chlorophorm extraction and buoyant gradient centrifugation. The most important step is disruption of the cell wall, which is very compact, by mechanical-chemical-enzymatic steps. This genetically faster detection and identification of phytopathogenic fungi will be applied without bioassay, meaning the faster protection of agriculture products.
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