Effect of phytohormones and some additives on tissue culture establishment and in vitro production of alkaloids from Arcangelisia flava Merr.
1987
Wandee Yannaphaisan (Silpakorn Univ., Bangkok (Thailand)) | Pannipha Chumsri | Krisna Kraisit
This is the first experiment being carried out on in vitro cultivation of Arcangelisia flava Merr. RT medium plus the combination of 2 mg/l NAA, 2 mg/l IBA and 1 mg/l kinetin is suitable for culturing callus of A. Flava Merr. This culture could produced berberine similar to nature. The production of berberine was increased in culture grown on medium plus either coconut milk or casein hydrolysate. However, the production of berberine was decreased in culture grown on medium plus citric acid. The production of berberine was high in callus cultured grown on coconut milk containing medium. The addition of manipulating agents such as tyrosine, manganese sulfate and aluminium sulfate in A. flava Merr. callus culture increased the berberine contents. Nevertheless, the callus could not grow on colchicine containing medium. A. flava Merr. in vitro culture produced highest berberine when either aluminium sulfate or tyrosine was added. The intensity of yellow color is the indication of amount of alkaloid production. The repeated selection of yellow callus produced high berberine contents.
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