Role of phylloplane and Rhizosphere microflora on pathogenicity of black leaf spot pathogen of peanut (Phaeoisariopsis personata)
1990
Nongnuch Kijjao
Phylloplane and rhizosphere microflora of peanut were isolated from plant and soil specimens obtained from three peanut planting areas (viz. National Corn and Sorghum Research Centre, NCSRC, Nakhon Ratchasima; Kampangsaen and Bangkhen Campuses of Kasetsart University). The leaf imprint method yielded maximum number of microorganisms. Besides, most of microorganisms were obtained from upper surface of peanut leaf. Effectiveness of these microflora as biological control agents (BCA) was tested by applying the spore suspension on to peanut leaves (detached leaf technique). Significant reduction of black leaf spot disease was found after BCA was applied in laboratory condition. The best BCA in laboratory are Fusarium oxysporum and Penicillium sp. Field trial on efficacy of BCA from peanut leaves, Bacillus subtilis was the best BCA to reduce black leaf spot whereas, no significant reduction of black leaf spot was obtained from the latter location.
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