Genetic structure of MDMV [maize dwarf mosaic virus] resistant and susceptible inbred lines of maize (Zea mays L.)
1995
Ignjatovic, D. | Tadic, B. | Ivanovic, D. (Maize Research Institute "Zemun Polje", Belgrade - Zemun (Yugoslavia))
In the study isozyme molecular markers were used for labelling the genomes of four resistant - A632, B84, 38-11, Pa405 and two susceptible - Mo17, Oh43 maize inbred lines. Coleoptile samples, obtained five days after germinating at 25 deg C in the dark, were analyzed. The biochemical method of horizontal electrophoresis on starch gel, followed by suitable histochemical dying, was used in the analysis of fifteen enzyme systems, covering eight chromosomes. No regularity could be established in the presence of various allelic forms within resistant and susceptible inbred lines. Most of the loci have the same allele present within all analysed lines. However, some of the loci are polymorphic. Isozyme markers of chromosome 6, Pgd1, Hex2, Idh2 and Mdh2, are polymorphic and as such can be used as informative (segregating) marker-genes in F2 or BC generations.
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