The requiremental difference of cytokinin and the endogenous cytokinin levels in trifoliate and yuzu
1995
Kato, Y. (Meiji Univ., Kawasaki, Kanagawa (Japan). Faculty of Agriculture) | Haga, K. | Sugiyama, T. | Akihama, T.
The requiremental difference of 6-benzylaminopurine was investigated by using hypocotyls from Trifoliate (Poncirus trifoliata Rif.), Yuzu (Citrus junos Sieb. ex Tanaka) and Valencia orange (Citrus sinensis Osbeck (f. valencia)). All Citrus species scarcely formed shoots in the formone free MS medium. In Trifoliate, the average numbers of shoots were 18.95 in 10.0 mg/l BA and 1.10 mg/l BA, while in Yuzu they were 1.03 and 2.1 respectively. Although the number was 1.45 in 0.01 mg/l BA, Valencia orange turned white and dead in 10.0 mg/l BA. Cytokinins were extracted from the roots of Trifoliate and Yuzu which had grown for two years. Cytokinin fraction was purified and then zeatin, ribosylzeatin and N6-isopentenyladenosine were identified and quantified by GC/MS. The content of zeatin was 1.27 microgram/100g fw. in Yuzu. It was about twice as much as that in Trifoliate and vice versa in the content of ribosylzeatin. N6-isopentenyladenosine in both plants was approximately the same level. Based on the fact that the requirement of cytokinin in Trifoliate was higher than that in Yuzu, it may by concluded that the long term endogenous cytokinin level becomes higher in the former than in the latter
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