Isolation, enumeration and identification of oil-degrading fungi in Pasig River [Philippines]
1995
Yap, R.A. | Halos, P.S.M. (Philippines Univ. Diliman, Diliman, Quezon City (Philippines). Inst. of Biology)
Water and soil samples were collected along the Pasig River-EDSA [Philippines] and analyzed for the presence of oil-utilizing fungi. A total of 16 fungi were isolated from Pasig River by plating technique using modified Czapek Dox agar medium with the diesel oil incorporated as the only carbon source. Of the 16 isolates, 14 utilized by hydrocarbon. The development of visible turbidity by the yeasts and mycelial growth and dry weights of the mold isolates in the amended basal salts medium correlated positively with hydrocarbon utilization. Of the 14 isolates, 12 were molds and 2 were yeasts. Except for the two yeast isolates, the rest belong to the Fungi Imperfecti. Three belong to the genus Aspergillus, two were Botrytis cinerea, while the rest belong to the genera Trichoderma, Aureobasidium, Botryotrichum, Goidanichiella, Humicola and Phialophora. One fungal and two yeasts isolates were not identified. The two isolates that were not able to utilize the diesel oil in the liquid medium belonged to the genus Aspergillus. Enumeration of population of the oil-degrading fungi showed that areas fairly exposed to hydrocarbon contamination contained a higher number of oil-degrading fungi then stations which were relatively free from oil contamination. Statistical analysis using Pearson product moment correlation showed that the prevalent pH had little effect on the population of oil-degrading fungi in Pasig River whereas temperature did not have any effect at all. However, variation in the number of colony-forming units (CFUs) is possible depending upon the prevalent weather conditions
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