Genotypic and non-genotypic factors influencing the anther culture and doubled haploid production in hexaploid triticale
1995
Balatero, C.H. | Darvey, N.L. | Luckett, D.J. (Philippines Univ. Los Banos, College 4031, Laguna (Philippines). Inst. of Plant Breeding)
Greenplant regeneration efficiencies of calli/embryoids derived from either semi-solid or liquid MC17 media were similar. Alleles causing high androgenetic response appear to be dominant over alleles controlling low callus/embryo induction based on the performance of different breeding populations (F1, F2 and backcrosses). Pretreatment of spikes with 0.05 percent colchicine three days prior to anther culture significantly improved callus/embryoid induction but slightly reduced the regeneration potential of the calli/embryoids. The use of chromosome doubling agent in triticale is deemed necessary owing to the low frequency of spontaneously DH plants (7-8 percent). The conventional method of doubling the chromosome number using colchicine (0.05 percent) significantly improved the overall level of DH plant production. Likewise, the use of donor plants capable of meiotic restriction increased the number of partially-fertile haploid plants
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