Chromosomal location of genes encoding glutelin polypeptides in rice
1996
Uemura, Y.J. | Satoh, H. | Ogawa, M. | Suehisa, H. | Katayama, T.C. | Yoshimura, A.
Two cultivars, Kinmaze (japonica) and IR36 (indica), were used for the electrophoretic characterization of glutelin polypeptides. The glutelin subunits were separated by isoelectric focusing electrophoresis (IEF) using a horizontal slab gel system, yielding 11 bands for the acidic (alpha) glutelin subunit and nine bands for the basic (beta) glutelin subunit. Almost all IEF bands of glutelin subunits were detected as single spots in the two-dimensional electrophoresis (IEF/SDS-PAGE), indicating that the IEF system is very useful for detecting mutants for glutelin polypeptides and for genetic analysis of these polypeptides. A wide variation of IEF bands for glutelin subunits was found among local rice cultivars. The genetic analysis of these bands demonstrate that the variations of glutelin polypeptides are caused by spontaneous mutation and that almost all of the individual bands are controlled by incomplete single dominant genes and some of the genes cluster on specific chromosomes. Restriction fragment length polymorphism analysis using recombinant inbred lines confirmed that the genes controlling the alpha-2 band are located into a cluster on chromosome 1 and genes controlling the alpha-3 and beta-2 bands are located in close linkage on chromosome 2
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