Studies on the possibilities of in vitro storage of grapevine explants by reducing the medium composition
1997
Ruseva, R. (Institut po Introduktsiya i Rastitelni Resursi, Sadovo (Bulgaria))
A study was conducted with grapevine cvs Bolgar, Pamid, Merlot and Hamburg to establish the survival rate and the storage duration of microcuttings in vitro by reducing the nitrogen compounds, the macro- and microelements and the total chemical composition of the medium. The results of the investigation showed that when reducing the nitrogen compounds alone to 1/4 of their content in medium, the microcuttings preserved their vitality to 70.66- 76.50%, but the time of their storage in vitro was rather short - up to 95.1 - 101.1 days. With the reduction of the macro- and microelement composition to 1/4, the time of storage increased, but the vitality of cultures decreased. The in vitro storage by reduced medium composition did not change the regenerative ability of the stored explants. When cultivated in a complete nutrient medium, they formed shoots to the 5th stem node for almost the same time as that of the unstored ones.
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