Somatic embryogenesis and regeneration of plantlets in common oak (Quercus robur L.)
1998
Tsvetkov, I. (Forest Research Institute, Sofia (Bulgaria))
Immature embryos of common oak (Q. robur L.) were used as initial explants for inducing the process of somatic embryogenesis. The best embryogenic response (53,3%) was registered on MS medium supplemented with either 1,0 mg/l BAP alone or in combination with 1,0 mg/l GA3. The embryos collected during the first week of July demonstrated highest embryogenic competence that rapidly decreased and got lost by the end of the period tested. Embryogenic cultures could be easily maintained in darkness by regular transfers on hormone-free MS medium. Some mature somatic embryos successfully germinated after being transferred to WPM medium with 0,2 mg/l BAP. Such embryos showed further development and converted into plantlets.
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