Superoxide dismutase isoenzyme spectra in the cattle and dog blood cells
1998
Karus, A. | Karus, V. (Estonian Agricultural Univ., Tartu (Estonia))
386 cattle and 21 dog blood samples were analysed. Blood cells were separated by centrifuging and destroyed by repeated freezing and defrosting in the presence of 5 x volume of bidist. water. Superoxide dismutase isoenzyme spectra were separated using the electrophoresis in the 7.5 per cent PAG and using the horizontal IEF. For determining the isoenzyme spectra the tetrazolium method was used. In cattle red blood cells was found SOD sub(A) in the only variant similar to SOD sub(A)2 observed in the dog red cells. In dog erythrocytes two allelic variants of the SOD sub(A) were found. This fact is an evidence of the existing of the multiple allelic forms of the superoxide dismutase in dog blood cells. In dog and cattle leukocytes both SOD sub(A) and SOD sub(B) were found. SOD sub(B) did not have allelic forms. SOD sub(A) isoenzyme had two allelic forms in dog leukocytes. Frequency of SOD sub(A)2 allele o,595 was higher than SOD sub(A)1 allele 0.405. Most frequent was SOD phenotype SOD sub(A)2-1/SOD sub(B)1. Phenotype SOD sub(A)1/SOD sub(B)1 was observed only twice. Both of them were Cocker Spaniels. SOD isoenzyme spectra in dog and cattle leukocytes with describing the respective phenotypes are shown in figures. Data about dog SOD phenotypes by dog breed are given in the table
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