Attachment of vero cells to corona-treated, plastic surfaces
1997
Ramsay, J. (Ecole Polytechnique de Montreal, Quebec (Canada)) | Rhazi, B. | Garnier, A. | Sapieha, S. | Chavarie, C. | Ramsay, B.
Corona treatment of polystyrene (PS) and polyethylene resulted in an increase in the interfacial energy (gamma-sv) of these surface, primarily due to an increase in the polar forces. The number of Vero cells which attached and the strength of attachment were higher on corona-treated surfaces, increasing as gamma-sv increased. Inoculum concentration did not affect the percentage of cells that attached. Experiments were performed in Dulbecco's modified Eagle's medium containing 10% fetal bovine serum cellular attachment was most likely preceded by adsorption of serum proteins. A minimum of 1 h of incubation under static conditions resulted in the maximum number of cells which attached and which remained attached after shear stress was applied. Experimental results with corona treated surfaces agree with the theoretical predictions for the free energy of adhesion of Vero sells in the medium used, indicating that interfacial energy plays an important role in attachment
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