Dietary safflower oil, compared to coconut oil, differently affects splenocyte functions in ovalbumin-sensitized rats, leading to elevation of the circulatory IgE
1997
Ju, H.R. (Kyushu Univ., Fukuoka (Japan). Faculty of Agriculture) | Wu, H.Y. | Ki, M. | Tachibana, N. | Sakono, M. | Nishizono, S. | Sugano, M. | Imaizumi, K.
Prostaglandin E2 (PGE2) and protein kinase C (PKC) affect proliferation of T cell and its subset which lead classical B cell isotype switching to immunoglobulin (Ig) E. In order to examine a mechanism(s) underlying different levels of circulating IgE in response to dietary saturated and polyunsaturated fats, production of PGE2 and activity of PKC were measured in splenocytes of ovalbumin (OVA)-sensitized rats. Safflower oil (SO) diet-fed rats, compared to coconut oil diet-fed rats, elevated serum levels of OVA-specific IgE and IgG2a and lowered the IgG1. Activity of PKC and concanavalin (Con) A-induced T-lymphoproliferation were lowered in splenocytes from SO diet-fed rats. Production of PGE2 was elevated splenocytes from SO diet-fed rats; however, this difference was disappeared when the cells were cultured with Con A. SO diet-fed rats increased proportion of linoleic acid but decreased the arachidonic acid in splenocyte phospholipids. We conclude that dietary polyunsaturated fatty acid is an important modulator of PGE2 production and PKC activity in splenic immune cells and it may play a role in the circulatory level of IgE
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