Detection of Pseudomonas solanacearum banana strains using DNA amplification techniques
1996
Ilagan, Y.A. | Raymundo, A.K. | Denny, T.P. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Biological Sciences)
A repetitive element from the banana (bugtok) strain of Pseudomonas solanacearum was cloned in the plasmid p Bluescript. The insert present in the clone was sequenced. Based on the sequence of the opposite ends, primers were designed and used in amplification reactions. Polymerase chain reaction experiments resulted to amplification of a 1.5 kb fragment among the bugtok and moko isolates (Philippine strains). No amplification was observed with non-banana strains and non-P. solanacearum strains. This PCR-based technique is a sensitive tool for the detection and identification of the pathogen in the field or in planting materials for quarantine purposes or for epidemiology studies
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