Screening and utilization of Pseudomonas for treatment of solvent-containing effluents
1997
Velasco, A.V.B.
Optimization of growth conditions for Pseudomonas putida 1337 showed a significant increase in viable cells from 10sup(7) and 10sup(10) cells/ml at 2 percent (v/v) initial level of toluene with 25 percent (v/v) initial inoculum concentration. In contrast, a slight growth from 10sup(7) to 10sup(8) cells/ml was attained when 2 percent (v/v) level of benzene was added initially in the medium with the same level of inoculum indicating tolerance of the organism towards the organic solvents at the indicated concentration. Increasing the initial concentration of solvents up to 10 percent (v/v) and decreasing the initial inoculum concentration to 10 percent (v/v) did not allow growth of the organism. There was no significant change in the pH of the medium after 6 days of incubation for benzene and toluence in all of the conditions tested. Effluent concentrations of 1/10, 1/2 (v/v) including effluents did not vary much in their support for growth of the organism. There was a significant drop in pH when undiluted effluents were used. Inoculation of toluene- and benzene- containing effluents with P. putida 1337 significantly reduced chemical oxygen demand by 2-fold. Toxicity test using the carter bottle method likewise resulted in a reduction of toxicity by 2-fold and 3-fold in treated toluence- and benzene-containing effluents, respectively. Determination of catechol as the first degradation product in toluene and benzene metabolism revealed the inability of the organism to degrade the compounds as no catechol was detected even at the onset of growth. Accordingly, these results indicate that the solvent-tolerant Pseudomonas putida 1337 had the characteristic of degrading solvent-containing effluents regardless of the presence or absence of benzene or toluene
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by University of the Philippines at Los Baños