Extraction and precipitation of pepsin from bovine gastric tissues
1997
Zia-Ur-Rehman | Shah, W.H. (PCSIR Labs., Lahore (Pakistan))
Optimum conditions for the extraction and precipitation of bovine pepsin from young buffalo were studied. Changes in pH, temperature and extraction time significantly affected the extraction of pepsin. Maximum pepsin activity (222 units g-1) was observed when minced gastric tissues were suspended in acidic solution of pH 2.2 at 40 Centigrade for 18 hr. Pepsin was precipitated from the extract by adding sodium chloride at the rate of 200g-1. Pepsin activity in camel, cow, buffalo and chicken gastric tissues, under these conditions was found to be 101, 141, 190 and 222 units respectively, while 172 units g-1 pepsin activity was observed in cases of sheep and goat. These gastric tissue samples of different animals were kept at -10 Centigrade for six months and a gradual decrease in pepsin activity by 18-68% was observed during storage.
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