Differences in O3-induced superoxide dismutase and glutathione antioxidant expression in O3, tolerant and sensitive trembling aspen (Populus tremuloides Michx.) clones
1997
Sheng, Y. | Karnosky, D.K. (Michigan Technological Univ., Houghton (USA). School of Forestry and Wood Products) | Podila, G.K.
Three aspen (Populus tremuloides Michx.) clones differing in ozone (O3) sensitivity were exposed to O3. The level of total glutathione, the activities of superoxide dismutase (SOD), glutathione reductase (GR) glutathione-S-transferase (GST) and mRNA levels of glutathione peroxidase (GPx), Mn SOD and cytosolic Cu/Zn SOD were simultaneously increased in the O3-tolerant clone 216 for the long-term exposure (100 nl/l O3 for 10 days or 2 months). For the O3-sensitive clone 259, only GPx mRNA increased markedly. Superoxide dismutase activity and cytosolic Cu/Zn SOD mRNA levels increased less than in the O3 intermediate and tolerant clones and total glutathione level and the activities of GR and GST were decreased relative to control following long-term O3 exposure. Following short-term O3 exposure (150 nl/l for 6 to 12 h), O3-induced SOD activity increased more rapidly in the O3-tolerant clone 216 than in the O3-sensitive clone 259 and intermediate clone 271. These results support the hypothesis that SOD and glutathione systems play integral roles in the protection of plants from oxidative stresses
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