Selection of a high anthocyanin-producing cell line from callus cultures of hybrid poplar (Populus alba L.x P. glandulosa Uyeki)
1997
Myung Suk Choi (Forest Genetics Research Inst., Suwon, (Korea Republic). Division of Biotechnology) | Young Goo Park
Reddish callus was visually selected from 15 callus lines based on color density. Anthocyanin synthesis ability was significantly different among 15 callus lines investigated. Line number 11 was considered as a reliable callus line for both high anthocyanin yield and rapid cell growth. Line number 11 frequently lost its ability for anthocyanin synthesis during repeated subculture and it did not provide reliable cell lines which produce high anthocyanin synthesis and cell growth. Callus derived from single cell colony was efficiently reselected by the cell aggregate selection method. The yield of anthocyanin from selected cell lines increased almost two times (from 0.8 to 1.7) after the final selection (after 17 weeks). Anthocyanin content markedly increased from the 7th week but cell growth slightly decreased with selections. The content of anthocyanin from homogeneous callus lines varied from 0.8 to 1.7 per 0.5 g fresh weight of cultures
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