Pathogenesis-related proteins in near-isogenic barley lines induced by powdery mildew infection
1997
Tamas, L. | Huttova, J. | Zigova, Z. (Slovak Academy of Sciences, Bratislava (Slovakia). Inst. of Botany)
Induction of pathogenesis-related (PR) proteins in intercellular spaces of near-isogenic barley leaves was examined after inoculation with a virulent and avirulent race of powdery mildew (Erysiphe graminis f. sp. hordei) 24, 48 and 72 h post-inoculation. Induction of two of the pathogen-induced proteins was ascertained 24 h post-inoculation. 72 h post-inoculation 7 to 8 major pathogen-induced proteins (bands on native PAGE) were identified. The accumulation pattern of PR proteins was very similar in both compatible and incompatible host-pathogen interaction but it was more expressive and appeared earlier at host-parasite incompatibility than at compatibility. These results confirm that the resistance gene plays a key role in early recognition of pathogenes as well as in early activation of the defence response
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