Isolation of DNA from some Thai rice varieties and cDNA from aromatic rice
1993
Duangporn Varasundharosoth | Lolita Meksongsee | Poontariga Harinasut (Kasetsart Univ., Bangkok (Thailand). Faculty of Science. Dept. of Biochemistry)
As part of a research work on rice storage protein genes for genetic manipulation to increase protein content of the popular aromatic rice KDML 105, seed proteins were fractionated from some Thai rice varieties to identify high protein varieties. The samples included the local red varieties GS 12052, GS 11012, GS 10244, GS 2683 together with KDML 105, since a variety of red rice has been reported to be high in protein content. The red variety GS 2683 showed the highest protein and glutelin contents. SDS-PAGE of the protein fractions revealed that the major protein subunits have the following sizes; the globulin to be 57 KD, prolamin as 16, 13 & 10 KD and glutelin as 23 KD. DNA was isolated from the above varieties as well as from some other recommended varieties i.e. RD 10, KPM 148, KTH 17 and NMS 4. Agarose gel electrophoresis of the DNAs separated these varieties into two groups of slightly differing band mobilities. Restriction fragment patterns of double digested DNA minipreparations will be presented. cDNA libray was initiated by preparing poly a mRNA from KDML 105 and cDNA synthesis was attempted.
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