Detection of mutation T-C 1843 in the RYR 1 gene, polymorphic protein and enzyme systems associated with malignant hyperthermia in Yugoslav autochthonous pig breeds
1997
Sarac, M. (Scientific Institute for Veterinary Medicine, Novi Sad (Yugoslavia)) | Gagrcin, M. | Jovanovic, S.
The analysis was carried on 30 pigs of two Yugoslav autochthonous breeds (15 pigs of the Bikovacka mangulica breed, and 15 pigs of the Moravka breed) of various ages, different sex and unknown porcine stress syndrome status (MH status). The results confirm the hypothesis of Fujii et al. (1991) that the cause of MH might be muatation at nt 1843 in the RYR 1 (ryanodine receptor 1) gene. The frequency of the RYR 1 (C) allele is 0.95 and RYR 1 (T) is 0.05. Polymorphism of GPI (glucosephosphate isomerase), PGD (6-phosphogluconate dehydrogenase) and PGM (phosphoglucomutase) were examined using agarose gel electrophoresis at pH 7.2. Using two-dimensional agarose at pH 5.4 in the first dimension and horizontal PAGE (pH 9.0) with 4, 8, 12 separation gel was examined the polymorphism of three different alfa-protease inhibitors, PI1, PO1A, PI2, and three other proteins, PO2 (postalbumin 2), Hpx (hemopexin) and Tf (transferrin). Swine resistant status is assotiated with 90 PGD (AA) genotype, 100 PO2 (FF) genotype, 60 PI2 (IS) and 60 Tf (BB) genotype. The GPI, PGM, PI1, PO1A and Hpx systems did not show any significant assotiations betwee stress resistnt status and high frequencies of certain genotypes.
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