Effect of vitamin C on disease resistance and immune response of hybrid catfish
1996
Wadsana Maneerat
First study was designed to investigate the beneficial effect of vitamin C (ascorbyl-2-polyphosphate) in enhancing the resistance of hybrid catfish (female Clarias macrocephalus X male Clarias gariepinus) to Aeromonas hydrophila. Hybrid catfish with average weight of 1.17+-0.05 gm were fed with diets containing 100 (control), 500, 1,000, 3,000 and 5,000 mg of vitamin C per kg diet for four weeks before being challenged with A. hydrophila. Result at 24 hrs. did not show any significant difference (P0.05) in rates of survival, but after 48 hrs. of challenging, catfish fed over 500 mg/kg diet did have significantly higher survival rate than the control group (P0.05), especially at 48 hrs, these differences were highly significant (P0.01). A second investigation was designed to determine the effect of vitamin C on specific immune response (antibody titer) to formalin-killed A. hydrophila vaccine. Hybrid catfish of 52.58+-1.83 gm in initial average weight were fed with experimental diets for four weeks, then they were immunized twice by intraperitoneal injection with 0.2 ml of vaccine. Antibody titers of all experimental groups reached peak after the first and the second immunization for one week. Statistical comparison of the titer amongst experimental groups within the same sampling period indicated no significant difference (P0.05). It can be concluded that hybrid catfish, fed with diet containing ascorbyl-2-polyphosphate more than 500 mg/kg diet for four weeks, developed significantly higher resistance to A. hydrophila (P0.05). However, vitamin C did not have any impact on specific immune response of this catfish.
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