Sunflower genotype reaction to direct and indirect organogenesis and somatic embryogenesis using three media and gamma ray treatment
1997
Encheva, J. | Ivanov, P. ("Dobroudja", General Toshevo (Bulgaria))
Immature zygotic embryos from nine inbred lines of sunflower (Helianthus annuus L.) were used as a donor material for the induction of direct organogenesis and somatic embryogenesis. A portion of the embryos was treated with Cs 137 gamma radiation before planting, at doses of 5, 10 and 15 Gy. Three induction media were used in the experiments - A0 by Freyssinet and Freyssinet (1988), E1 (modified A0 medium) and TPM by Wilcox et al. (1988). The regeneration was strongly increased with the dose of 5 Gy in line Z-8-A in the medium A0, followed by the dose of 10 Gy in the lines 1395 Rf in A0 and RNA-801 in E1. For the three media and all genotypes the dose of 5 Gy in the medium A0 appear to be most appropriate for increasing the frequency of plant regeneration.
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