Methods for determination of alpha-amylase activity and its influence to spring wheat quality
2000
Ingver, A. | Koppel, R. | Annamaa, K. (Jogeva Plant Breeding Inst., Jogeva (Estonia))
Precipitation during harvest induces germination in already physiologically ripe kernel. The result is breakdown of the kernel protein and starch reserves, which reduces yield potential and quality. Most widely used indirect method for determination of enzyme alpha-amylase activity is falling number method. To measure level of sprouting of wheat kernels in early stages of breeding work, new methods have to be worked out. Joint project between CPRO-DLO (The Netherlands) and Jogeva Plant Breeding Institute (Estonia) aims to work out a new indirect method for assessing alpha-amylase activity in small amount of kernels. Gel-diffusion method is promising rapid method for screening a large number of samples, including single grains or half grains in early stages of a breeding program
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