Effects of irradiation and incubation of palm oil empty fruit bunch medium on the growth of rice mushroom (Volvariella volvaceae)

English. Study on the effect of irradiation and time of incubation of composted palm oil empty fruit bunch (EFB) were conducted in growth chamber in the plastic bag as growth medium. Treatments of experiment were consisted of sterilization of composted EFB growth medium by autoclave heating and gamma irradiation. Autoclave heating were conducted at 2.0 kg/cm2 for 40 minutes and gamma irradiation at the dose of 0, 15, 30, and 45 kGy. After sterilization, EFB medium were inoculated using mushroom seed of V. volvaceae. Time of incubation of EFB medium after inoculation was 2 and 4 weeks before mushroom harvesting. Growth chamber was maintained at 28-35 deg. C room temperature and humidity 80 percent. Parameters of the experiment were determined on mushroom analysis i.e. fat, protein, and starch content and weight of mushroom. Analysis of EFB medium after incubation were determined i.e. total fiber and percentage of rendement. Result of the experiment showed that dose of 45 kGy affected on mushroom fat content 0.52 percent which was decreasing regards to the delay of incubation period. Irradiation treatment did not affect EFB fat content compared to non irradiated EFB (0.82 percent). Irradiation treatment and incubation on EFB medium were not increasing on mushroom protein content. Mushroom starch content was obtained on the dose of 15 kGy and 4 weeks of incubation period 3.09 and 3.13 percent respectively. The dose of 45 kGy and autoclave heating were decreasing total fiber of EFB medium 29.29 and 28.02 percent respectively. The dose of 45 kGy on EFB medium with 2 weeks of incubation period produced weight of mushroom 30.92 and 27.73 g/bag respectively

Indonesian. Telah dilakukan studi pengaruh iradiasi dan inkubasi media tandan kosong kelapa sawit (TKKS) dalam kantong plastik pada ruang inkubator pertumbuhan. Perlakuan yang diberikan ialah sterilisasi panas dengan autoklaf dan dengan iradiasi gamma pada media TKKS. Sterilisasi media TKKS dilakukan pada tekanan 2,0 kg/cm2 selama 40 menit, sedangkan iradiasi dilakukan pada dosis 0, 15, 30, dan 45 kGy. Semua media diinokulasi dengan bibit jamur merang kemudian diinkubasi selama 2 dan 4 minggu dalam ruang inkubator pada suhu 28-35 derajat C dan kelembaban 80 persen. Parameter percobaan yang diuji ialah kadar lemak dan protein jamur merang dan media TKKS, kadar pati jamur, kadar serat kasar media TKKS dan rendemen, dan bobot jamur merang. Hasil percobaan menunjukkan bahwa perlakuan iradiasi 45 kGy pada media TKKS dapat menghasilkan kadar lemak jamur merang sebanyak 0,52 persen, tetapi semakin lama masa inkubasi, semakin turun kadar lemak jamur merang. Perlakuan iradiasi tidak mempengaruhi kadar lemak media TKKS dan perlakuan tanpa inkubasi menghasilkan kadar lemak tertinggi yakni sebanyak 0,82 persen. Perlakuan iradiasi dan inkubasi pada media TKKS tidak meningkatkan kadar protein jamur merang. Kadar pati jamur merang tertinggi dihasilkan pada perlakuan iradiasi 15 kGy sebanyak 3,09 persen dan 3,13 persen dengan masa inkubasi 4 minggu. Perlakuan iradiasi 45 kGy menurunkan kadar serat kasar media TKKS hingga 29,29 persen dan 28,02 persen dengan perlakuan autoklaf. Perlakuan iradiasi 45 kGy pada media TKKS dapat menghasilkan bobot jamur sebesar 30,92 gram/kantong dan 27.73 gram/kantong dengan masa inkubasi 2 minggu