[Agronomic effects in peanut (Arachis hypogaea L.) associated with three regeneration methods]
1999
Utomo, S.D. (Universitas Lampung, Bandar Lampung (Indonesia). Fakultas Pertanian)
Plant regenerations is a critical step in most plant genetic transformation methods, but they may cause alterations generally described as somaclonal variation. The objective of this study was to compare three regeneration methods by evaluating 20 agronomic traits among peanut lines derived from R0 plants. One hundred ninety two R1: 2 lines were derived from 48 R0 plants, 12 R0 per culture methods, i.e., embryonic axis culture (EAC), immature embryo culture (IEC), immature leaflet culture (ILC), and sexual reproduction (control). Families were grown at two locations in North Carolina, USA in the summer 1994. The trait means of IEC-derived families were significantly different from those of the control for leaflet length and width, main stem height, and total length of 20 seeds. The trait means of ILC-derived families were also significantly different from those of the control for number of primary branches and leaflet length. IEC, ILC, and EAC appeared to induce alterations for four, two, and zero traits, respectively. However, based on observation of other 15 traits including pod and seed yield, the three regeneration methods are equally suitable as regeneration methods for peanut transformation
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