In vitro culture of ilang-ilang [Cananga odorata (Lam.) Hook F. and Thomas]
1999
Malabayabas, M.D. | Del Rosario, A.G. (Philippine Rice Research Inst., Munoz, Nueva Ecija (Philippines))
The optimum conditions for the in vitro culture of ilang-ilang shoot tip explants was investigated. A sterilization protocol involving a series of pre-sterilization cleaning of shoot tip explants and the use of 0.3 percent sodium hypochlorite was established. Murashige and Skoog's (MS) Medium or Woody Plant Medium (WPM) with 1.0 to 2.0 mg/l benzyladenine (BAP) and 0.1 to 0.5 mg/l napthaleneacetic acid (NAA) was found to be suitable for callus formation. High frequency shoot bud differentiation was obtained in MS medium containing 2.0 mg/l BAP with 0.1 to 0.2 mg/l NAA. Differentiated shoot buds transferred to WPM with 2 mg/l BAP in combination with 0.1 and 0.5 mg/l NAA exhibited normal shoot growth while those transferred to MS medium containing the same growth regulators formed protocorm-like structures on the adaxial surface of the leaves. High frequency and better root formation was obtained in WPM basal medium supplemented with 0.5 mg/l indolebutyric acid. Regenerated plantlets were successfully in greenhouse conditions. Plantlet regeneration from ilang-ilang shoot tip explants was achieved by indirect organogenesis
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