Genetic diversity analysis of Luffa species based on seed protein profile using SDS-PAGE [
1997
Tolentino, M.I.S. | Laude, R.P. | Dela Vina, A.C. (Philippines Univ. Los Banos, College, Laguna (Philippines). Inst. of Biological Sciences)
The taxonomic relationship of and genetic diversity in smooth gourd, Luffa cylindrica and ridged gourd, L. acutangula based on total seed protein profile using SDS-PAGE was determined. A total of 12 major bands were resolved. Bands 1, 2, 3 and 4 were the relatively high molecular weight proteins, bands 5 and 6 were in the intermediate region and the last six bands belong to the relatively low molecular weight group Bands 4, 5, 7, 9 and 10 were present in all the accessions. Ten band patterns (BP) were observed on the 215 accessions. These were BP1 (compose of bands 4, 5, 7, 9 and 10); BP2 (2, 4, 5, 7, 8, 9, and 11); BP3 (2, 3, 4, 7, 8, 9 and 11); BP 4 (3, 4, 5, 7, 9, and 10); BP 5 (3, 4, 5, 6, 7, 9, and 10); BP6 (3, 4, 5, 6, 7, 9, 10 and 11); BP 7 (2, 3, 4, 5, 7, 9, and 10); BP8 (8, 4, 5, 7, 9, and 10); BP9 (1, 2, 3, 4, 5, 7, 9 and 10) and BP10 (1, 2, 3, 4, 5, 7, 9, 10, 11 and 12). Four band patterns namely: BP1, 7, 8, and 9 were common in the two species. On the other hand, BP4, 5 and 10 were specific to L. cylindrica while BP 2, 3 and 6 were specific to L. acutangula. Similarity indices among accessions of both species were high, 80.0 percent L. cylindrica and 71.1 percent for L. acutangula. Domestication of the smooth gourd may have contributed to its lesser diversity than ridged gourd. A similarity index of 68.2 percent between the two species suggests that the genome and ridged gourds do not differ very much from each other
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