Activity of acid phosphatase in black tiger prawn (Penaeus monodon)
1994
Prasat Kittakoop | Menasveta, P. (National Center for Genetic Engineering and Biotechnology. Chulalongkorn Univ., Bangkok (Thailand). Marine Biotechnology Research Unit)
Vitamin C and stable forms of its derivatives, ascorbyl-2-monophosphate, ascorbyl-2-polyphosphate, and ascorbyl-2-sulphate, are commonly used as a vitamin C source in feed industry. There is a number of work on bioaviability of these forms of vitamin C on various animals, particularly on aquatic animals that are economically important such as, fish and shrimp. Mostly studies on bioaviability of these vitamin C derivatives showed that the vitamers could provide high growth rate and survival rate to animals, however, there is few reports on their biochemistry in animals, i.e., how these derivatives are biochemically converted to an active form (ascorbic acid) inside a body of animals. This work demonstrated that an activity of the crucial enzyme, acid phosphatase, which catalyzes the conversion of ascorbyl-2-monophosphate to ascorbic acid, was present in an hepatopancreas of black tiger prawn (Penaeus monodon). An enzymatic assay was based on the reduction of a colour of 2,6-dichlorophenol-indophenol when ascorbic acid released from a substrate (ascorbyl-2-monophosphate) by the enzymatic activity. High activity of acid phosphatase up to 2.4 micro mole ascorbic acid produced per hour was found in a dialysed enzyme extract.
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