Membrane enzymes as possible biosenzor for lead monitoring in water
1997
Nikezic, G. (Institut za nuklearne nauke Vinca, Beograd (Yugoslavia). Laboratorija za molekularnu biologiju i endokrinologiju) | Horvat, A. | Todorovic, S. | Vasic, V. | Vujisic, Lj.
In our earlier work, we have shown that enzymes from rat brain synaptosomal membranes, Na(+)/K(+)-ATPase and Mg(2+)-ATPase, are promising biological components of a biosensor for lead detection. In this work, we represent our results of investigation with the same enzymes as biological components for the biosensor in presence of Hg(2+) ions in water. It was established that IC50 for Na(+)/K(+)-ATPase and Mg(2+)-ATPase is 6.9 and 5.5 x 10E-6 M, and the percentages of inhibition are 96% and 77% respectively. We concluded that these enzymes could be the base for developing biosensors for the presence of Hg(2+) ion in water. Since these enzymes maintain a stable activity for a longer period of time, they could be appropriate as components of biosensors for monitoring water quality.
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