Protein changes associated with somatic embryogenesis in cotton (Gossypium hirsutum L.)
1999
Vandenhout, H. | Swennen, R. (Katholieke Univ. Leuven (Belgium). Lab. of Tropical Crop Improvement) | Demot, R. | Schoofs, G.
Protein samples were extracted from callus at different developmental stages of the non-commercial Cotton variety Coker 312, Coker 100 and Delta Pine 15 (Gossypium hirsutum L.). Proteins were separated by two-dimensional gel electrophoresis (IEF- PAGE-SDS) and silver stained. Gels were analysed with the ImageMaster software (Pharmacia-Biotech). Protein patterns differed with the level of tissue development. The aim of the study is to provide a tool for the identification of proteins specific for somatic embryogenesis and regeneration.
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