Plant regeneration from mature Avena tissue explants
1999
Hassan,G. | Zipf,A. | Sharma,G.C. | Wesenberg,D.
Successful plant transformation is dependent upon a reliable plant regeneration system.In cereals, immature embryos are the explants of choise but are inconvenient due to their temporal nature and maintenance requirements.The effects of three different auxins, 2,4-D, dicamba, and picloram, on two mature tissue explant types, mature embryo and hypocotyl, of three Avena genotypes, GAF/Park, Park, and 88Ab3073, were evaluated for callus formation, somatic embryogenesis (SE), and plant regeneration capabilities.Although plant number was significantly correlated with SE number, it was not absolutely linked to a priori somatic embryogenesis, especially for 88AB3073 explants.
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