Plant regeneration from scutella of immature embryos of diverse sorghum genotypes
2000
Sairam,R.V. | Seetharama,N. | Shyamala,T. | Devi,P.S.
We compared two different culture media for high frequency callus induction and plant regeneration from cultured immature embryos (excised scutella) of 14 sorghum genotypes.Embryogenic calli were induced in 10-15 days after the embryos were plated on LS medium with 0.25 mg/l zeatin and 0.5 mg/l 2,4-D.For induction of embryogenic callus, this media was better than the other with 2,4-D alone.Regeneration was achieved by transferring embryonic calli to MS medium containing 2 mg/l NAA, 0.5 mg/l BAP and 0.1 mg/l GA3.Both callusing and regenerating ability varied (10-100%) among the genotypes.Popular seed parents 296 B and BTx 623 showed 100% callus induction and regeneration.
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