Overproduction of dnaJ in Escherichia coli improves in vivo solubility of the recombinant fish-derived transglutaminase
1998
Yokoyama, K. (Ajinomoto Co. Inc., Tokyo (Japan)) | Kikuchi, Y. | Yasueda, H.
The overexpression of red sea bream (Pagrus major)transglutaminase (TGase, EC 2.3.2.13) in Escherichia coli mostly leads to the accumulation of biologically inactive enzyme. Although the solubility of the gene products could by improved by cultivation at a lower temperature (26-28 degrees C), most of he synthesized TGase was still in the form of insoluble aggregates. The effects of overproduction of molecular chaperons on the intracellular solubility of newly produced recombinant TGase were examined. The overexpression of dnaK or groES/EL did not improve solubility. However, DnaJ greatly increased the solubility of the recombinant TGase, resulting in active enzyme in the presence of calcium ions. Co-expression of dnaK along with dnaJ further increased the content of soluble TGase. Under our experimental conditions, supplementation with both DnaJ and DnaK elevated the TGase activity in the producer cells by roughly 4-fold, compared with the control strain cultured at 30 degrees C. Thus, we found that DnaJ is important in controlling the solubility of protein overproduced in E. coli
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