Identification of gibberellins and 9,15-cyclogibberellins in developing apple [Malus pumila] seeds
1996
Oyama, N. (Tokyo Univ. (Japan)) | Yamauchi, T. | Yamane, H. | Murofushi, N. | Agatsuma, M. | Pour, M. | Mander, L.N.
Endogenous gibberellins (GAs) and GA-related compounds at two different developmental stages of apple seeds (Malus domestica cv. McIntosh) were analyzed by gas chromatography-mass spectrometry. From the seeds at ca. 10 and/or 14 weeks after full-bloom, the following GAs and 9,15-cyclogibberellins (9,15-cyclo-GAs) were identified by a comparison of their mass spectra and Kovats retention indices with those of authentic specimens: GA4, GA7, GA9, GA12, GA17, GA19, GA20, GA25, GA34, GA35, GA45, GA53, GA54, GA61, GA62, GA63, GA70, GA73, GA80, GA84, GA88, 3-epi-GA4,3-epi-GA54, and 3-epi-GA63, 9,15-cyclo-GA9, 1-beta-hydroxy-9,15-cyclo-GA9, 2-beta-hydroxy-9.15-cyclo-GA9, 3-alpha-hydroxy-9,15-cyclo-GA9, 3-beta-hydroxy-9,15-cyclo-GA9, and 11-beta-hydroxy-9,15-cyclo-GA9. The major components in the seeds at ca. 10 weeks after full-bloom were GA4, GA7, GA9, GA17, GA35, GA54, GA62, GA80, and GA84, and the those in the seeds at ca. 14 weeks after full-bloom were GA17, GA25, GA45, GA62, GA63, GA80, GA84, 9,15-cyclo-GA9, and 1-beta, 3-beta-, and 11-beta-hydroxy-9,15-cyclo-GA9. New GA number (GA103-108) have been allocated to 9,15-cyclo-GA9, and 1-beta, 2-beta-, 3-beta-, 3-alpha, and 11-beta-hydroxy-9,15-cyclo-GA9, respectively (1-beta- and 3-alpha-hydroxy-9,15-cyclo-GA9 were identified previously as fern anteridiogens, although GA numbers have not previously been assigned to these compounds). This is the first report of the co-occurrence of GA73 and 9,15-cyclo-GAs in higher plants
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