Induction of cryoprotectants and lower supercooling points in the eggs of the silkworm, Bombyx mori, by a synthetic diapause hormone
1999
Adachi, T. (Kyoto Inst. of Technology (Japan)) | Ohmaki, K. | Furusawa, T.
To clarify the physiological role of polyols (sorbitol and glycerol), which accumulate in Bombyx eggs, experiments were performed to investigate the relationships between supercooling point (SP) and the accumulated polyols in inherent diapause eggs and in artificial diapause eggs which were induced by the injection of a synthetic diapause hormone (DH) into the pupae of a non-diapause egg producer. In the inherent diapause eggs, the SP fell with the increase of sorbitol and glycerol during diapause initiation, and rose with the decrease of polyol during the termination of diapause. During the maintenance of diapause, the SP remained at an almost constant level at -29 degrees C, although the polyol content increased during this period. In non-diapause eggs used as a control, sorbitol content was at a trace level, and glycerol content was below 0.8 mu-moles/100 eggs during embryonic development. The SP of these eggs rose from -24 degrees C just after oviposition to -20.5 degrees C just before hatching. The SP of the synthetic DH-induced diapause eggs, which accumulated sorbitol, glycerol, alanine and proline, was almost the same level (-29 degree C) as that of the inherent diapause ones, and rose with the decreasing of polyol content when they were chilled continuously to 5 degrees C. The relationship between amino acid (alanine and proline) and SP is discussed
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