New molecular markers linked with the high shoot regeneration capacity of the wild tomato species Lycopersicon chilense
1998
Takashina, T. (Yamagata univ., Tsuruoka (Japan). Faculty of Agriculture) | Suzuki, T. | Egashira, H. | Imanishi, S.
The goal of this research was to identify molecular markers that could assist in the selection of high shoot regeneration capacity from the progeny of a cross between a tomato cultivar and a "peruvianum-complex". The BC1F1 and BC2F1 generations between a tomato cultivar, "Kyoryoku Ogata Toko" (KOT) and L. chilense PI128644 (together with both the parents and the F1 hybrids) wee used as materials. BC2F1 generation was developed by a cross between KOT and a self-compatible BC1F1 plant that had high shoot regeneration capacity. Root explants of KOT cultured on an MS medium supplemented with 1 mg/l zeatin riboside did not form any shoots, while those of the PI128644 and the F1 hybrids showed a high shoot regeneration (SR) rate of more than 80%. The BC1F1 generation could be divided into 3 groups (0%, less than 30% and more than 80%) for SR rate. The BC2F1 generation could be divided only into 2 groups; about 70% of the plants had 0% SR rate and the other 30% had a continuous and non-peak frequency distribution up to 100%. The segregation of the RAPD markers and the acid invertase gene marker (inv-chi) which were specific to the PI128644, wee evaluated in Bc11 and BC2F1 to determine a possible relationship between these markers and the ability to regenerate shoots. The Mann-Whitney test showed that the RAPD markers, OPA02-1, OPA20-3 and inv-chi were mostly present in the plants with the high shoot regeneration capacity. It was suggested that these three molecular markers are closely linked to the high shoot regeneration gene of L. chilense PI128644
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